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- W2387365493 abstract "Objective Establishment and characterization of healthy donor's γδT cell clones.Methods γδT cells were cloned by limiting dilution after positive sorting with 60Co irradiated allogeneic PBMC as feeder cells.Flow cytometry analysis and molecular biology technique were then used to identify γδT cell clones.MTT assay was used to verify their proliferation after incubated with epitope peptides recognized by γδT cells.Results A γδT cell clone had been established.The subtype of this clone was V γ9 V δ2 without expression of CD4 and CD8.Further studies indicated that epitope peptide EP6 could not only specifically bind to γδT cell clone but also trigger the proliferation of γδT cell clone.Conclusion A healthy donor's γδT cell clone was successfully established,which laid a solid foundation for further study on γδT cells." @default.
- W2387365493 created "2016-06-24" @default.
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- W2387365493 date "2009-01-01" @default.
- W2387365493 modified "2023-09-26" @default.
- W2387365493 title "Establishment and characterization of a healthy donor's γδT cell clone" @default.
- W2387365493 hasPublicationYear "2009" @default.
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