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- W2387395343 abstract "Objective To screen and identify the specific aptamer for cyclosporin A(CsA) by SELEX protocol.Methods A single stranded DNA(ssDNA) random library with 78 nucleotides in length containing 35 random sequences was constructed.Biotin-streptavidin-horseradish peroxidase system was applied with magnetic beads as screening media to determine the binding affinity between ssDNA library of each round and cyclosporin A and to screen the aptamers for CsA.PCR products of the last round of screen were cloned and sequenced,and their primary and secondary structures were analyzed using related software.Result After 10 rounds of screen,an increasing tendency of binding affinity between aptamers and cyclosporin A was present.19 randomly selected aptamer clones could be classified as 5 families based on the homology of the primary structure and the hairpin loop was the main motif in the prediction of the secondary structure.Conclusion We can obtain the specific aptamers for cyclosporin A by the establishment of SELEX technique." @default.
- W2387395343 created "2016-06-24" @default.
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- W2387395343 date "2007-01-01" @default.
- W2387395343 modified "2023-09-26" @default.
- W2387395343 title "Establishment of SELEX technique for screening DNA aptamer for cyclosporina A" @default.
- W2387395343 hasPublicationYear "2007" @default.
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