Matches in SemOpenAlex for { <https://semopenalex.org/work/W2387455213> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2387455213 abstract "Objective:A novel tuberculosis DNA vaccine integrating siRNA,fusional antigen and hIL-12 triple expression units was constructed in our laboratory.Methods:To evaluate the independent expression of the three expression units,two eukaryotic expression plasmid pVAX1-siRNA-PVAE[EGFP]-hIL12 with TB fusional gene Ag85B-ESAT6(PVAE) and enhanced green fluorescent protein(EGFP),and pVAX1-siEGFP-PVAE[EGFP]-hIL12 with siRNA coding sequence targeting EGFP instead of Mcl-1L were constructed.Then two plasmids were used to transfer human embryonic kidney 293 cells.Based on EGFP report gene,it was demonstrated that siRNA expression unit and fusional antigen gene were independently expressed.Results:The hIL12 expression at 48h and 72h post transfection were also detected by ELISA analysis up to 1571.63pg/ml and 2392.25pg/ml respectively in the cell culture fluid.Conclusion:The results demonstrated that the novel DNA vaccine with siRNA,TB fusional antigen and hIL12 three expression units in the same plasmid frame is successfully constructed and independently expressed in eukaryotic cells.It laid a foundation for further animal model study on anti-tuberculosis effects of this novel DNA vaccine." @default.
- W2387455213 created "2016-06-24" @default.
- W2387455213 creator A5028978808 @default.
- W2387455213 date "2008-01-01" @default.
- W2387455213 modified "2023-09-23" @default.
- W2387455213 title "The Detection of Triple Expression of Tuberculosis DNA Vaccines on the Cell Level" @default.
- W2387455213 hasPublicationYear "2008" @default.
- W2387455213 type Work @default.
- W2387455213 sameAs 2387455213 @default.
- W2387455213 citedByCount "0" @default.
- W2387455213 crossrefType "journal-article" @default.
- W2387455213 hasAuthorship W2387455213A5028978808 @default.
- W2387455213 hasConcept C104317684 @default.
- W2387455213 hasConcept C142613039 @default.
- W2387455213 hasConcept C147483822 @default.
- W2387455213 hasConcept C153911025 @default.
- W2387455213 hasConcept C159047783 @default.
- W2387455213 hasConcept C167508917 @default.
- W2387455213 hasConcept C22744801 @default.
- W2387455213 hasConcept C33161422 @default.
- W2387455213 hasConcept C40767141 @default.
- W2387455213 hasConcept C54009773 @default.
- W2387455213 hasConcept C54355233 @default.
- W2387455213 hasConcept C552990157 @default.
- W2387455213 hasConcept C81885089 @default.
- W2387455213 hasConcept C86803240 @default.
- W2387455213 hasConceptScore W2387455213C104317684 @default.
- W2387455213 hasConceptScore W2387455213C142613039 @default.
- W2387455213 hasConceptScore W2387455213C147483822 @default.
- W2387455213 hasConceptScore W2387455213C153911025 @default.
- W2387455213 hasConceptScore W2387455213C159047783 @default.
- W2387455213 hasConceptScore W2387455213C167508917 @default.
- W2387455213 hasConceptScore W2387455213C22744801 @default.
- W2387455213 hasConceptScore W2387455213C33161422 @default.
- W2387455213 hasConceptScore W2387455213C40767141 @default.
- W2387455213 hasConceptScore W2387455213C54009773 @default.
- W2387455213 hasConceptScore W2387455213C54355233 @default.
- W2387455213 hasConceptScore W2387455213C552990157 @default.
- W2387455213 hasConceptScore W2387455213C81885089 @default.
- W2387455213 hasConceptScore W2387455213C86803240 @default.
- W2387455213 hasLocation W23874552131 @default.
- W2387455213 hasOpenAccess W2387455213 @default.
- W2387455213 hasPrimaryLocation W23874552131 @default.
- W2387455213 hasRelatedWork W2349034691 @default.
- W2387455213 hasRelatedWork W2350120007 @default.
- W2387455213 hasRelatedWork W2352517994 @default.
- W2387455213 hasRelatedWork W2355649761 @default.
- W2387455213 hasRelatedWork W2355688609 @default.
- W2387455213 hasRelatedWork W2355690490 @default.
- W2387455213 hasRelatedWork W2361123973 @default.
- W2387455213 hasRelatedWork W2362053331 @default.
- W2387455213 hasRelatedWork W2362754024 @default.
- W2387455213 hasRelatedWork W2368337115 @default.
- W2387455213 hasRelatedWork W2372227229 @default.
- W2387455213 hasRelatedWork W2382798072 @default.
- W2387455213 hasRelatedWork W2383011939 @default.
- W2387455213 hasRelatedWork W2387691506 @default.
- W2387455213 hasRelatedWork W2393049065 @default.
- W2387455213 hasRelatedWork W2398823130 @default.
- W2387455213 hasRelatedWork W2420122227 @default.
- W2387455213 hasRelatedWork W3211804290 @default.
- W2387455213 hasRelatedWork W44468211 @default.
- W2387455213 hasRelatedWork W62330710 @default.
- W2387455213 isParatext "false" @default.
- W2387455213 isRetracted "false" @default.
- W2387455213 magId "2387455213" @default.
- W2387455213 workType "article" @default.