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- W2387492064 abstract "Purpose To develop a high-performance liquid chromatographic method for the determination of polyamine in human saliva.Methods The derivation with dansyl chloride was followed by normal-phase chromatography with a mobile phase of acetone /chloroform(2∶98)at a flow rate of l mL/min and with the detection wavelength set at 425nm.Results The standard curve was linear over the concentration range of 50-1 000 nmol/mL for putrescine and spermine with correlation coefficient 0.999 1 and 0.999 2.The recoveries were 94.65% with RSD 1.6% and 90.48% with RSD 2.1% for putrescine and spermine,respectively.Conclusion The present study provided a sensitive,accurate and reproducible method for the determination of polyamine levels in human saliva." @default.
- W2387492064 created "2016-06-24" @default.
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- W2387492064 date "2008-01-01" @default.
- W2387492064 modified "2023-09-23" @default.
- W2387492064 title "Determination of putrescine and spermine content in human saliva by HPLC with precolumn derivation" @default.
- W2387492064 hasPublicationYear "2008" @default.
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