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- W2387506425 abstract "Objective To establish the model of staphylococcus epidermidis(SE) biofilms(BF) on the surface of polyvinyl chloride(PVC) material,and evaluate the value of confocal laser scanning microscope(CLSM) and scanning electron microscope(SEM) in studying the configuration of SE-BF. Methods The in vivo dynamic study was performed after SE-RP62A BF formation on the surface of PVC material culture in TBS at 37 ℃ at 6,12,18,24,30,48 h respectively. The thickness of BF,colony number,percentage of vital bacterial and scanning picture of SE-RP62A biofilm on PVC of each group were measured by CLSM,and the configuration of SE biofilm was observed by SEM. Results It was shown that SE BF had highly organized structure with multi-cell colony population. A lot of vital and dead bacterium gathered densely in BF. The BF formation on the surface of PVC material was a dynamic process,the peak of bacterial adhesion needed 12 ～ 18 hours,and formed mature BF in 24 hours. There was significant difference of the percentage of vital bacterial among interior,middle and outer layers(P 0.05) of material. Conclusion The SE BF had a complicated structure,combined performing CLSM and SEM is the ideal method for study BF on PVC material." @default.
- W2387506425 created "2016-06-24" @default.
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- W2387506425 date "2007-01-01" @default.
- W2387506425 modified "2023-09-22" @default.
- W2387506425 title "Study on the structure of bacterial biofilm on PVC surface" @default.
- W2387506425 hasPublicationYear "2007" @default.
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