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- W2387561567 abstract "Objective:A method of molecular typing of Lactobacillus was established and optimized by pulsed field gel electrophoresis(PFGE). Methods:PFGE was used in the genetic typing study of Lactobacillus to optimize the factors influencing the typing of PFGE including different restriction endonuclease,different reaction concentration and reaction time. With these optimized factors,typing research was conducted to the 22 reference strains of Lactobacillus. Results:Restriction endonucleases Ascl was selected in this study with the optimized condition to be reaction concentration of 30U/150μL,reaction time of 4.5 hours and pulse time of 1~15s. By this technique,five electrophoretypes were distinguished in five reference strains of the different species,but the Lactobacillus within a species could not be distinguished by the PFGE. Conclusion:PFGE could be used to classify and identify Lactobacillus." @default.
- W2387561567 created "2016-06-24" @default.
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- W2387561567 date "2011-01-01" @default.
- W2387561567 modified "2023-09-28" @default.
- W2387561567 title "Optimization in molecular typing of Lactobacillus by pulsed field gel electrophoresis" @default.
- W2387561567 hasPublicationYear "2011" @default.
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