Matches in SemOpenAlex for { <https://semopenalex.org/work/W2387633902> ?p ?o ?g. }
Showing items 1 to 61 of
61
with 100 items per page.
- W2387633902 abstract "Objective To invest the inhibitory effect of tumor growth and the expression of vascular endothelial growth factor (VEGF) by acetoacetate extractive of Celastrus orbiculatus Thunb in orthotropic human liver carcinoma nude mice model. Methods Human HCC HepG2 cells transduced with red fluorescent protein (RFP-HepG2) were inoculated into the liver of BALB/c nude mice. The tumor-bearing nude mice were randomly divided into five groups: control group (G1 group), Oxaliplatin positive control group (G2 group, 25 mg/kg), COT low dose group (G3 group, 20 mg/kg), COT high dose group (G4 group, 40 mg/kg) and COT early treatment group (G5 group, 20 mg/kg). G5 group of mice was treated with COT p.o. from day 2 post tumor implantation. All other mice were treated from day 20 post tumor implantation. The growth of xenografted tumors was traced weekly by in vivo fluorescence imaging technology. Real time whole body fluorescence imaging was performed to measure tumor growth during the study. At the end of treatment, all of mice were killed and tumor tissue were collected. VEGF expression in the tumors collected at autopsy was analyzed by immunohistochemical staining. VEGF mRNA expression in the tumors collected at autopsy was analyzed by reverse transcription-polymerase chain reaction (RT-PCR). Results The COT significantly inhibited the growth of xenografted tumors. The inhibitory effect of COT in G5 group was superior to those in G3 group and G4 group, which was almost close to G2 group. VEGF protein was found to be expressed in the cytoplasm of all liver cells and the cell membrane of some liver cells. Average optical density (D) of the tumor VEGF expression was significantly reduced by high-dose COT and early COT treatment, compared to G1 group and G3 group (t = 4.657, 6.440, 7.712, 8.900; P = 0.0037, 0.0031, 0.001, 0.001). The tumors from early treatment with COT and oxaliplatin-treated mice had significantly lower VEGF expression levels compared to all other COT-treated mice. Consistent with VEGF protein expression by immunohistochemistry, the tumor VEGF mRNA expression was significantly reduced by G4 group and G5 group, compared to G1 group and G3 group (t = 6.320, 7.563, 12.907, 12.545; P = 0.0002, 0.0003, 0.001, 0.001). Conclusions The acetoacetate extractive of C. articulatus Thunb possess the effect of anti-tumor in vivo. The COT inhibits the growth of tumor. Inhibit VEGF expression by COT may inhibit angionesis and then may contribute in part to the inhibition of tumor growth." @default.
- W2387633902 created "2016-06-24" @default.
- W2387633902 creator A5070538645 @default.
- W2387633902 date "2013-01-01" @default.
- W2387633902 modified "2023-09-22" @default.
- W2387633902 title "Expression of vascular endothelial growth factor by acetoacetate extract from celastrus orbiculatus thunb in orthotropic human liver carcinoma nude mice model" @default.
- W2387633902 hasPublicationYear "2013" @default.
- W2387633902 type Work @default.
- W2387633902 sameAs 2387633902 @default.
- W2387633902 citedByCount "0" @default.
- W2387633902 crossrefType "journal-article" @default.
- W2387633902 hasAuthorship W2387633902A5070538645 @default.
- W2387633902 hasConcept C142724271 @default.
- W2387633902 hasConcept C150903083 @default.
- W2387633902 hasConcept C167734588 @default.
- W2387633902 hasConcept C204232928 @default.
- W2387633902 hasConcept C207001950 @default.
- W2387633902 hasConcept C2777025900 @default.
- W2387633902 hasConcept C2778019345 @default.
- W2387633902 hasConcept C2779012181 @default.
- W2387633902 hasConcept C502942594 @default.
- W2387633902 hasConcept C71924100 @default.
- W2387633902 hasConcept C86803240 @default.
- W2387633902 hasConceptScore W2387633902C142724271 @default.
- W2387633902 hasConceptScore W2387633902C150903083 @default.
- W2387633902 hasConceptScore W2387633902C167734588 @default.
- W2387633902 hasConceptScore W2387633902C204232928 @default.
- W2387633902 hasConceptScore W2387633902C207001950 @default.
- W2387633902 hasConceptScore W2387633902C2777025900 @default.
- W2387633902 hasConceptScore W2387633902C2778019345 @default.
- W2387633902 hasConceptScore W2387633902C2779012181 @default.
- W2387633902 hasConceptScore W2387633902C502942594 @default.
- W2387633902 hasConceptScore W2387633902C71924100 @default.
- W2387633902 hasConceptScore W2387633902C86803240 @default.
- W2387633902 hasLocation W23876339021 @default.
- W2387633902 hasOpenAccess W2387633902 @default.
- W2387633902 hasPrimaryLocation W23876339021 @default.
- W2387633902 hasRelatedWork W2044446332 @default.
- W2387633902 hasRelatedWork W2055281446 @default.
- W2387633902 hasRelatedWork W2122723283 @default.
- W2387633902 hasRelatedWork W2347877122 @default.
- W2387633902 hasRelatedWork W2352190709 @default.
- W2387633902 hasRelatedWork W2362414011 @default.
- W2387633902 hasRelatedWork W2364740277 @default.
- W2387633902 hasRelatedWork W2368675268 @default.
- W2387633902 hasRelatedWork W2368792506 @default.
- W2387633902 hasRelatedWork W2375772118 @default.
- W2387633902 hasRelatedWork W2385759658 @default.
- W2387633902 hasRelatedWork W2388007691 @default.
- W2387633902 hasRelatedWork W2390171725 @default.
- W2387633902 hasRelatedWork W2390399325 @default.
- W2387633902 hasRelatedWork W2518682401 @default.
- W2387633902 hasRelatedWork W3006665190 @default.
- W2387633902 hasRelatedWork W3028682169 @default.
- W2387633902 hasRelatedWork W3030919467 @default.
- W2387633902 hasRelatedWork W3031293433 @default.
- W2387633902 hasRelatedWork W3032664339 @default.
- W2387633902 isParatext "false" @default.
- W2387633902 isRetracted "false" @default.
- W2387633902 magId "2387633902" @default.
- W2387633902 workType "article" @default.