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- W2387637010 abstract "Enterokinase(EK,EC 3.4.21.9)is an important enzyme widely used in gene engineering.In this study,the inhibitory effects of several denaturant guanidine hydrochloride,thiourea and urea on the activity of rEK for the hydrolysis of Gly-Asp-Asp-Asp-Asp-Lys-β-naphthylamide were investigated by the monitoring the change of fluorescence.The results indicated that guanidine hydrochloride,thiourea and urea had strong inhibitory effect on the activity of rEK,with the concentrations of 0.025,0.080 and 0.750 mol/L,respectively leading to 50% enzyme activity lost.Research showed that the mechanism of guanidine hydrochloride,thiourea and urea effecting on rEK activity were reversible,belonging to competetive type inhibitory.The inhibition constants of guanidine hydrochloride,thiourea and urea were respectively 0.015,0.060 and 0.553 mol/L." @default.
- W2387637010 created "2016-06-24" @default.
- W2387637010 creator A5049049288 @default.
- W2387637010 date "2011-01-01" @default.
- W2387637010 modified "2023-09-23" @default.
- W2387637010 title "Effects of Different Denaturant on Recombinant Bovine Enterokinase" @default.
- W2387637010 hasPublicationYear "2011" @default.
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