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- W2387639211 abstract "[Objective and Method]In order to detect and analyze the ASSVd in precocious pear trees, low molecular weight RNAs were extracted from tender leaves and shoots of precocious pear trees which were collected from No.223 Farming Herding Regiment in Hejing County,Xinjiang Uygur Autonomous Region. Then the samples were detected by reverse transcription - polymerase chain reaction(RT - PCR).[Result]The results showed:(1) Two of the precocious pear trees were infected with Apple scar skin viroid(ASSVd) and the 2 isolates(accession numbers JX861258 - JX861259) had over 86%nucleotide sequence identity with previously published sequences in the GenBank.(2) In situ RT - PCR further confirmed the existence of ASSVd in the leaf tissues and indicated that ASSVd was mainly distributed in the nucleus.[Conclusion]There was no geographic and variety differentiation between ASSVd isolates.Optimized detection methods of RT - PCR and In situ RT - PCR were established,thus providing the basis for the rapid identification of ASSVd in fruit trees." @default.
- W2387639211 created "2016-06-24" @default.
- W2387639211 creator A5027485227 @default.
- W2387639211 date "2013-01-01" @default.
- W2387639211 modified "2023-09-25" @default.
- W2387639211 title "Detection and Sequence Analysis of the Apple Scar Skin Viroid Isolated from Pear Trees in Xinjiang" @default.
- W2387639211 hasPublicationYear "2013" @default.
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