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- W2387662926 abstract "Objective To study the isolation and purification procedure of TI from Houttuynia cordata Thunb.Methods The optimal criteria of TI extraction were 45 ℃,30 times of solvent volume,pH 7.0 and 2 h.The combination of ammonium sulphate precipitation and DEAE-cellulose column chromatography was used to purify the TI.Results The specific activity peak of TI occurred in the part of 45%～60% (NH4)2SO4 precipitation,and was consequently purified by DEAE-cellulose column gradient elution,and the activity of TI could be detected.Conclusion The HCTI can be purified with DEAE-cellulose column,and the molecular weight of HCTI is determined by SDS-PAGE as 28 KDa." @default.
- W2387662926 created "2016-06-24" @default.
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- W2387662926 date "2010-01-01" @default.
- W2387662926 modified "2023-09-26" @default.
- W2387662926 title "The Extraction and Purification of Trypsin Inhibitor from Houttuynia cordata Thunb" @default.
- W2387662926 hasPublicationYear "2010" @default.
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