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- W2387663634 abstract "Objective To apply Padlock rolling-circle amplification(Padlock-RCA) for the analysis of in situ gene expression in individual cells during differentiation.Methods Retinoic acid induced differentiation of mouse teratocarcinoma F9 model was used,Nanog mRNA expression in F9 cells before and after the induced differentiation was analyzed with Padlock-RCA and compared with result of RT-PCR.Results Padlock-RCA can efficiently detect the level of Nanog mRNA expression in single cells.Nanog specific Padlock-RCA products were significantly decreased in F9 cell following retinoic acid induction,and confirmed with RT-PCR(P0.05).Conclusion We have successfully applied Padlock-RCA to analyze differential Nanog mRNA expressions before and after the induced differentiation in single cell,and provided a reliable method for molecular and functional analyses of gene expression in single cell or cell subpopulations." @default.
- W2387663634 created "2016-06-24" @default.
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- W2387663634 date "2011-01-01" @default.
- W2387663634 modified "2023-09-22" @default.
- W2387663634 title "In situ analysis of gene expression in single cell with Padlock-RCA" @default.
- W2387663634 hasPublicationYear "2011" @default.
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