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- W2387684759 abstract "Objective:To construct 3 eukaryotic expression vectors of short hairpin RNA(shRNA) against livin gene and then transfect into HCT-8/V cells,and to pave the way to research that gene-silencing of livin in HCT-8/V on trait of vincristine resistance.Methods:Three pairs of oligonucleotide based on livin mRNA sequences were synthesized and annealed to produce transcription templates,and then linked with linear plasmid pGCsi-H1/Neo/GFP to generate siRNA eukaryotic expression vectors.Plasmids were extracted following DH5|a strains were transformed.Then insert sequences were identified and recombinants were transfected HCT-8/V cells.Results:The insert sequences were identical with designed shRNA template sequences according to sequencing.Expression of green fluorescent protein in transfected HCT-8/V was observed by fluorescence microscopy.Conclusions:The recombinant vectors were established and transfected into HCT-8/V cells successfully,HCT-8/V cell with expression vectors of livin siRNA was screened by G418 and proliferated." @default.
- W2387684759 created "2016-06-24" @default.
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- W2387684759 date "2007-01-01" @default.
- W2387684759 modified "2023-09-24" @default.
- W2387684759 title "Construction and transfection of expression vectors of siRNA for inhibitor of apoptosis protein" @default.
- W2387684759 hasPublicationYear "2007" @default.
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