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- W2387732288 abstract "ABM: To investigate the effects of human recombininant macrophage colonly - stimulating factor (M - CSF) and protein kinase C inhibitor, staurosporine (STA) , on the binding of macrosialin of the mouse peritoneal macrophages (MPM) to ox - LDL. METHODS: MPM was disrupted by using ultrasonic pulse method after preincubation of M - CSF and STA. The plasma membrane proteins were separated by SDS - PAGE under nonreducing condition. The separated proteins were transferred to a nitrocellulose membrane. The ligand blotting and immunoblotting were used. The effects of M - CSF and STA on expression of cell surface receptor were observed by means of autoradiography. RESULTS: Preincubation with medium containing neuraminidase dramatically decreased the binding of macrosialin to [125I] - ox - LDL [(2.45 ± 0.46) μg/g cell protein vs (58.38 ± 1.78) (μg/g cell protein]. When pretreated with M - CSF, macrosialin bound predominantly to ox - LDL. The Bmax of [125] - ox - LDL to macrosialin increased when macrophages were treated with M- CSF [(322.77± 12.54) vs (453.59± 15.39) μg/g] . Meanwhile, the dissociation constant of treated group showed little changes [(29.06±2.87) vs (28.26 ±4.10) mg/L]. The similar result was found when macrosialin was pretreated with STA, a protein kinase C inhibitor. The Bmax of [125I] - ox - LDL to macrosialin increased [(264.76± 11.29) vs (362.40± 15.31) μg/g]. The dissociation constant of treated group showed little changes either [(17.43±2.98) vs (15.10±2.67) mg/L]. CONCLUSION: The results indicate that M - CSF and STA enhance the binding of macrosialin to ox - LDL by increasing the cellular surface receptor number." @default.
- W2387732288 created "2016-06-24" @default.
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- W2387732288 date "2005-01-01" @default.
- W2387732288 modified "2023-09-25" @default.
- W2387732288 title "Increased effects of M - CSF and staurosporine on the binding of macrosialin of MPM to ox - LDL" @default.
- W2387732288 hasPublicationYear "2005" @default.
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