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- W2387739731 abstract "Glutathione-S-transferases (GSTs) were a class of the important xenobiotics-metabolizing enzymes in insects. In this research, a 771 bp GST-Omega1 gene (encoded 256 amino acids) was cloned by RT-PCR from the midgut of Bombyx mandarina for exploring the expression profiling of enzyme GSTs in eukaryotic expression system. Analysis by Conserved Domains online tool of NCBI website indicated that the deduced amino acid sequence was comprised of the omega family conserved sequences with one Cys residue and eight GSH binding sites. GST-Omega1 was then cloned into the pFastBacHT b vector, and the recombinant vector was transformed into DH10Bac competent cells to obtain the bacmid DNA. Spodoptera frugiperda Sf9 cells were transfected with the complexes of bacmid DNA and lipofectin to get recombinant baculovirus. The SDS-PAGE and Western blotting results of the expression of recombinant protein showed that a specific band was detected around 33 kD, consistent with the expected size of the fusion protein. The purified target protein by His-Bind resin could catalyze the universal GST substrate CDNB, and exhibited enzymatic activity K_m 2.81 μmol/L and V_ max 2.70 μmol/(mg·min)." @default.
- W2387739731 created "2016-06-24" @default.
- W2387739731 creator A5021094177 @default.
- W2387739731 date "2007-01-01" @default.
- W2387739731 modified "2023-09-25" @default.
- W2387739731 title "Expression of an omega class glutathione S-transferase gene from Bombyx mandarina in the Sf9 cell" @default.
- W2387739731 hasPublicationYear "2007" @default.
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