Matches in SemOpenAlex for { <https://semopenalex.org/work/W2387777423> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W2387777423 abstract "According to the gene sequences of 16 S r RNA gene in Gen Bank, one pair of specific primer was designed for amplifying the specific fragments of 16 S r RNA gene. Then 16 S r RNA gene amplified by PCR was cloned into p MD-18 T vector and it was used as positive standard. After optimization of annealing temperature and primer concentration, a SYBR Green I Real-time Quantitative PCR was established for detection of Flavobacterium columnar. The melting curve analysis using SYBR Green I dye showed one specific peak, no primer-dimers peak was observed. No amplification was amplified from Aeromonas hydrophila, Edwardsiella tarda, Pseudomonas fluorescens, Citrobacter freundii by the SYBR Green I Realtime Quantitative PCR. The PCR kit was highly sensitive in 12 copies/μL DNA. The results revealed that the SYBR Green I Real-time Quantitative PCR kit was sensitive, specific and it could be used to detect F. columnar in clinical samples." @default.
- W2387777423 created "2016-06-24" @default.
- W2387777423 creator A5083163675 @default.
- W2387777423 date "2014-01-01" @default.
- W2387777423 modified "2023-09-25" @default.
- W2387777423 title "A SYBR GreenI Real-time Quantitative PCR kit for detection of Flavobacterium columnar of giant salamander" @default.
- W2387777423 hasPublicationYear "2014" @default.
- W2387777423 type Work @default.
- W2387777423 sameAs 2387777423 @default.
- W2387777423 citedByCount "0" @default.
- W2387777423 crossrefType "journal-article" @default.
- W2387777423 hasAuthorship W2387777423A5083163675 @default.
- W2387777423 hasConcept C104317684 @default.
- W2387777423 hasConcept C153911025 @default.
- W2387777423 hasConcept C178790620 @default.
- W2387777423 hasConcept C185592680 @default.
- W2387777423 hasConcept C2776832872 @default.
- W2387777423 hasConcept C2777563447 @default.
- W2387777423 hasConcept C2780968160 @default.
- W2387777423 hasConcept C48023723 @default.
- W2387777423 hasConcept C49105822 @default.
- W2387777423 hasConcept C54355233 @default.
- W2387777423 hasConcept C84133368 @default.
- W2387777423 hasConcept C86803240 @default.
- W2387777423 hasConceptScore W2387777423C104317684 @default.
- W2387777423 hasConceptScore W2387777423C153911025 @default.
- W2387777423 hasConceptScore W2387777423C178790620 @default.
- W2387777423 hasConceptScore W2387777423C185592680 @default.
- W2387777423 hasConceptScore W2387777423C2776832872 @default.
- W2387777423 hasConceptScore W2387777423C2777563447 @default.
- W2387777423 hasConceptScore W2387777423C2780968160 @default.
- W2387777423 hasConceptScore W2387777423C48023723 @default.
- W2387777423 hasConceptScore W2387777423C49105822 @default.
- W2387777423 hasConceptScore W2387777423C54355233 @default.
- W2387777423 hasConceptScore W2387777423C84133368 @default.
- W2387777423 hasConceptScore W2387777423C86803240 @default.
- W2387777423 hasLocation W23877774231 @default.
- W2387777423 hasOpenAccess W2387777423 @default.
- W2387777423 hasPrimaryLocation W23877774231 @default.
- W2387777423 hasRelatedWork W1987000848 @default.
- W2387777423 hasRelatedWork W2076928291 @default.
- W2387777423 hasRelatedWork W2128408897 @default.
- W2387777423 hasRelatedWork W2324161450 @default.
- W2387777423 hasRelatedWork W2324597869 @default.
- W2387777423 hasRelatedWork W2347351150 @default.
- W2387777423 hasRelatedWork W2359389555 @default.
- W2387777423 hasRelatedWork W2361013444 @default.
- W2387777423 hasRelatedWork W2362095823 @default.
- W2387777423 hasRelatedWork W2367484401 @default.
- W2387777423 hasRelatedWork W2369872108 @default.
- W2387777423 hasRelatedWork W2372488960 @default.
- W2387777423 hasRelatedWork W2374666610 @default.
- W2387777423 hasRelatedWork W2374933054 @default.
- W2387777423 hasRelatedWork W2377643777 @default.
- W2387777423 hasRelatedWork W2378538675 @default.
- W2387777423 hasRelatedWork W2386446751 @default.
- W2387777423 hasRelatedWork W2388468714 @default.
- W2387777423 hasRelatedWork W2394381572 @default.
- W2387777423 hasRelatedWork W3039101253 @default.
- W2387777423 isParatext "false" @default.
- W2387777423 isRetracted "false" @default.
- W2387777423 magId "2387777423" @default.
- W2387777423 workType "article" @default.