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- W2387788983 abstract "The gene coding CDK2 specific peptide were synthesized with PCR and cloned into C-terminal of GST expression vector pGEX-6P-1. The fusion proteins, GST-CDK2 peptide, were expressed in E.coli(BL21) by adding 0.1 mmol/L IPTG and purified with Glutathione Sepharose 4B. The rabbit was immunized with the mixtures of purified fusion proteins and complete Freund’s adjuvants. The sera of immunized rabbit were obtained and then analyzed by Western Blot. The result showed that these polyclonal antibodies in the sera can recognize the human CDK2 proteins from HeLa cells. These data suggested that the sera can be used as specific antibodies against CDK2 proteins in studying the role of CDK2 in the process of cell cycle and apoptosis." @default.
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- W2387788983 date "2007-01-01" @default.
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- W2387788983 title "Preparation and identification of the polyclonal antibodies against cyclin-dependent kinase 2" @default.
- W2387788983 hasPublicationYear "2007" @default.
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