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- W2387814639 abstract "Objective To study the effects of dexamethasone on the expression of estrogen receptor in osteoblast of different months old female rats. Methods A large amount of osteoblasts were isolated and cultured from the trabecular bone of different months old female Wister rats with trypsin and collagenase Ⅱdigestion method. Osteoblast was confirmed by the morphology alkaline phosphatase staining and estrogen receptor was detected by immunohistochemisty and western-blot. Results The osteoblasts isolated by the method above mentioned showed positive by the methods of morphology alkaline phosphatase staining and immunohistochemisty. The result of western-blot showed that the ER in osteoblast of female rat expression was higher on the newborn and 8-month-old rats than on the 2-,4-and 12-month-old ones. The level of ER expression in the osteoblasts which was affected by the dexamethasone was higher on the 4-,8-and 12-month-old female rats and lower on the newborn and 2-month-old ones, compared with the ER level of the osteoblasts without the effect of dexamethasone. Conclusions The level of ER expression in the osteoblast of female rat is different in rats of different age. ER expression's up-regulation or down-regulation in osteoblast of rat by dexamethasone at the concentration of 1×10~ -1 mol/L is correlated with the age of rats." @default.
- W2387814639 created "2016-06-24" @default.
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- W2387814639 date "2007-01-01" @default.
- W2387814639 modified "2023-09-26" @default.
- W2387814639 title "Effects of dexamethasone on the expression of estrogen receptor in osteoblast of different months old female rat" @default.
- W2387814639 hasPublicationYear "2007" @default.
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