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- W2387836099 abstract "To develop more universal molecular markers for grasses, rich genetic information of bamboo plants and 14 pairs of primers were designed according to the conserved regions of the single-copy orthologous(COSⅡ) genes of the Poaceae(grass) family. Then, polymerase chain reaction(PCR) was used to test five bamboo accessions. Also, Primer 1 and Primer 2 were used to sequence parts of the amplified products, and single nucleotide polymorphisms(SNP) sites were validated with the PCR-restriction fragment length polymorphism(RFLP) test. Based on different band styles amplified with the 14 primer pairs, genetic distance was calculated with NT sys 2.10e software and a cluster analysis of five species: Phyllostachys edulis, Ph. viridiglaucescens,Ph. nigra, Ph. violascens, and Ph. vivax f. aureocaulis were conducted. Results showed that all primers exhibited specific PCR products in at least one sample. Seven pairs of primers were able to amplify the products in tested samples with 13(92.9%) pairs of primers exhibiting polymorphic PCR products in all samples. Sequencing showed that Primer 1 and Primer 2 were homologous. These sequences were amplified with the same primer and existed at SNP sites. Some SNP sites led to early termination of the amino acid sequence, and some resulted in changes of restriction endonuclease sites. Results of the PCR-RFLP test corresponded with the sequencing. The cluster analysis indicated that Ph. edulis and Ph. viridiglaucescens were closely clustered, and Ph. nigra was the most isolated. Overall, this classification coincided with morphological classification; so, thesemolecular markers were suitable for application in genetic analyses and other related research with Phyllostachys." @default.
- W2387836099 created "2016-06-24" @default.
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- W2387836099 date "2014-01-01" @default.
- W2387836099 modified "2023-09-25" @default.
- W2387836099 title "Universal genetic markers for the Poaceae family" @default.
- W2387836099 hasPublicationYear "2014" @default.
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