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- W2387862884 abstract "In order to solve the problem of polyhexose interfering the genomic DNA extraction of Zizyphus jujuba Mill. , 3 methods, the conventional CTAB, TE3D and improved CTAB were compared. The results showed that the conventional CTAB method was difficult to get rid of polyhexose completely; the TE3D method could remove polyhexose effectively, but extraction rate was very low, DNA browned and degraded seriously; the improved CTAB method was an effective way to solve all kinds of problem, DNA concentration was up to 1000 ng/μL, degradation was light and impurity was little, A260/A280 was 1.80 or so. When tested through the DNA-AFLP fingerprinting, they could meet experiment requirement perfectly. In addition, the key steps and main features of the improved CTAB method are discussed." @default.
- W2387862884 created "2016-06-24" @default.
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- W2387862884 date "2006-01-01" @default.
- W2387862884 modified "2023-09-28" @default.
- W2387862884 title "Extraction methods of high quality DNA from Zizyphus jujuba" @default.
- W2387862884 hasPublicationYear "2006" @default.
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