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- W2387883906 abstract "AIM To establish a method of cultured neuron injury models by high air pressure for the study of injured cell group or single cell in the central nervous system (CNS). METHODS Neuron injuring model was produced by using high air pressure and assessed by nuclear uptake of the fluorescent dye propidium iodide (PI), which is excluded from cell with intact membranes however the dye penetrates injured cells and binds to DNA in the nuclei which then fluo resces red. Lactate dehydrogenase (LDH) enzyme was measured spectrophotometrically. RESULTS High air pressure of 1.0 MPa caused neuron injury and got the ideal injuring model by the air pressure of 1.5 MPa. CONCLUSION This model is appropriate for studying the biochemistry and pathophysiology of neuron injury." @default.
- W2387883906 created "2016-06-24" @default.
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- W2387883906 date "2002-01-01" @default.
- W2387883906 modified "2023-09-23" @default.
- W2387883906 title "Injury model of central neuron by air pressure in vitro" @default.
- W2387883906 hasPublicationYear "2002" @default.
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