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- W2387900851 abstract "The objective of this paper is to study the expression of mBD2 in MDCK cell and its activity for against IAV.The established eukaryotic expressing plasmid pcDNA3.1/mBD2 was transfected into MDCK cell by PolyFect transfection Reagent.The positive clone with pcDNA3.1/mBD2 was screening with G418.Expression of target gene and peptide in transfected cells was detected with RT-PCR and the immunofluorescence assays,respectively.The activity of anti-IAV of transfected cells was measured with TCID50.Additionally,pcDNA3.1/mBD2 was injected into mice to observe its effect against IAV in vivo.The results showed that pcDNA3.1/mBD2 plasmid was transfected successfully and expressed stably in MDCK.The TCID50 on transfected cells decreased 77.98 times compared with control group,and it had distinct difference between the experiment and control groups(P0.05).The protection rate for the mice immuned with pcDNA3.1/mBD2 after challenged with lethal IAV was 55.55%.These results suggested mBD2 has a clear activity for against IAV in vivo and in vitro,and it established a foundation for studying mBD2 against IAV." @default.
- W2387900851 created "2016-06-24" @default.
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- W2387900851 date "2011-01-01" @default.
- W2387900851 modified "2023-09-28" @default.
- W2387900851 title "Expression on mouse β-defensin 2 in MDCK and its activity against influenza A virus" @default.
- W2387900851 hasPublicationYear "2011" @default.
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