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- W2387922068 abstract "Objective To construct eukaryotic expression vector and to express in COS-7 cells human interferon-λ3 protein that possesses characteristic of antivirus. Methods RNA was taken from lung carcinoma cells A549 which have been stimulated by vesicular stomatitis virus, through reverse-transcribing-polymerase chain reaction throughout DNA was obtained, they were ligated with plasmid pcDNA3. 1/myc-his( - )A, forming recombination pcDNA3. 1A-hIFN-λ3 and expressing in COS-7 cells. The effect of antivirus of expressed product was investigated with a cytopathic effect reduction assay. Results The hIFN-λ3 sequence was identified by use of PCR, restriction endonucleases analysis and DNA sequencing. The hIFN-λ3 sequence was defined not only correct in orientation but also conformed with sequence reported by GeneBank. The hIFN-λ3 protein that has characteristic of antivirus was expressed in COS-7 cells. Conclusion In this study hIFN-λ3 gene sequence has been colonized successfully that transiently expressed in COS-7 cells and hIFN-λ3 protein is defined to possess characteristic of antivirus, which has laid the foundation for hIFN-λ3 gene therapy in clinic." @default.
- W2387922068 created "2016-06-24" @default.
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- W2387922068 date "2005-01-01" @default.
- W2387922068 modified "2023-09-23" @default.
- W2387922068 title "Gene Clone and Expression of Human Interferon-λ3" @default.
- W2387922068 hasPublicationYear "2005" @default.
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