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- W2387948257 abstract "In In this paper,genetic transformation of potato cultivar Desiree was conducted utilizing the Agrobacterium tumefaciens strain EHA105 that contains the binary vector pYLTAC7 with the phosphinothricin resistance(Bar) and hygromycin phosphotransferase(hpt) genes.The time of pre-cultivation,concentrations of Cefotaxime,Hygromycin and Herbicide Basta were studied.Our results suggested that: The optimal pre-cultivation time was 2d;Cefotaxime(Cefo),at the concentration of 300mg/L,was able to effectively suppress the growth of Agrobacterium after co-cultivation;The optimal concentrations for Basta and Hygromycin were,respectively,0.6 and 15mg/L and The presence of the Bar gene in the genomic DNA of transgenic plants was verified by PCR amplification.A stem transformation and regeneration system was devised for potato Desiree using Basta or Hygromycin as a selective marker." @default.
- W2387948257 created "2016-06-24" @default.
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- W2387948257 date "2008-01-01" @default.
- W2387948257 modified "2023-09-23" @default.
- W2387948257 title "Studies on Application of Two Genetic Marking Substances in Agrobacterium-Mediated Transformation of Potato" @default.
- W2387948257 hasPublicationYear "2008" @default.
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