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- W2387948981 abstract "Objective To explore the change of reparable cells in scalded tissue and to establish a model of apoptosis due to heat injury in vitro. Methods Human dermal fibroblasts were cultured in vitro, cells in Dulbecco's modified Eagle's medium cultured in a water saturated atmosphere. Dulbecco's modified Eagle's medium was supplied with 10% or 5% calf serum. Cells were stimulated at 43℃,45℃ and 48℃ for 10 minutes, 30 minutes and 40 minutes, respectively. DNA gel electrophoresis, Hoeschst 33258 fluorescence staining and transmission electron microscope were used to determine the condition of apoptosis. In the control group, fibroblasts were incubated at 37℃ for 30 minutes. Results Fibroblast apoptosis occurred at 10, 30 and 40 minutes under water bath at 43℃ and 45℃. Fibroblasts in Dulbecco's modified Eagle's medium was supplied with 5% calf serum and stimulated at 45℃ for 10 minutes. There were a lot of apoptotic cells. However, in the cultured fibroblasts stimulated at 48℃ for more than 30 minutes, there was mass cellular necrosis, on the contrary, there were no significant apoptotic cells in the control group. Conclusions Apoptosis in fibroblasts can be successfully induced by heat injury in vitro. This model can be used to study the biological character of fibroblasts under wound healing in vitro." @default.
- W2387948981 created "2016-06-24" @default.
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- W2387948981 date "2002-01-01" @default.
- W2387948981 modified "2023-09-25" @default.
- W2387948981 title "Heat injury fibroblasts: a useful model to study wound healing in vitro" @default.
- W2387948981 hasPublicationYear "2002" @default.
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