Matches in SemOpenAlex for { <https://semopenalex.org/work/W2387956917> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2387956917 abstract "The effective clinical application of human telomerase promoter (hTERT promoter) in tumor gene therapy is limited by insufficient tumor-targeting transcription activity at -34 E-box of its core promoter. To test whether it can be enhanced by additioanl E-boxes,a wild-type core hTERT (WT-hTERT) promoter (-268 to -10 upstream of ATG start codon) and a modified hTERT (Mod-hTERT) promoter (with a triplet of E-boxes ligated to 3' WT-hTERT) were synthesized. Their fusions with enhanced green fluorescent protein (EGFP) or luciferase were constructed and transfected into 293FT,HepGⅡ,SGC7901,U2OS,and the primary culture human fibroblasts (PHF),respectively. The results showed that in the Mod-hTERT-driven cells,EGFP expression could be observed in hTERT-positive cells like 293FT,HepGⅡ,and SGC7901 cells,but not in hTERT-negative cells as U2OS and PHF. The luciferase activity in Mod-hTERT transfected cells was significantly higher than that of the WT-hTERT in the hTERT positive cell lines of 293FT,HepGⅡ,and SGC7901(P0.01),but lower in the hTERT-negative cell lines of U2OS (P0.01). In PHF,no significant difference was found (P0.05). Our study indicates that multiple E-boxes improve the tumor-targeting transcription activity of the core hTERT promoter." @default.
- W2387956917 created "2016-06-24" @default.
- W2387956917 creator A5087000148 @default.
- W2387956917 date "2009-01-01" @default.
- W2387956917 modified "2023-09-25" @default.
- W2387956917 title "hTERT Core Promoter Modified by E-boxes Element and Transcriptional Activity Assay in vitro" @default.
- W2387956917 hasPublicationYear "2009" @default.
- W2387956917 type Work @default.
- W2387956917 sameAs 2387956917 @default.
- W2387956917 citedByCount "0" @default.
- W2387956917 crossrefType "journal-article" @default.
- W2387956917 hasAuthorship W2387956917A5087000148 @default.
- W2387956917 hasConcept C101762097 @default.
- W2387956917 hasConcept C104317684 @default.
- W2387956917 hasConcept C111335760 @default.
- W2387956917 hasConcept C125593758 @default.
- W2387956917 hasConcept C138885662 @default.
- W2387956917 hasConcept C142613039 @default.
- W2387956917 hasConcept C150194340 @default.
- W2387956917 hasConcept C153911025 @default.
- W2387956917 hasConcept C179926584 @default.
- W2387956917 hasConcept C185592680 @default.
- W2387956917 hasConcept C202751555 @default.
- W2387956917 hasConcept C41895202 @default.
- W2387956917 hasConcept C502942594 @default.
- W2387956917 hasConcept C54009773 @default.
- W2387956917 hasConcept C54355233 @default.
- W2387956917 hasConcept C55493867 @default.
- W2387956917 hasConcept C81885089 @default.
- W2387956917 hasConcept C86803240 @default.
- W2387956917 hasConcept C94581717 @default.
- W2387956917 hasConceptScore W2387956917C101762097 @default.
- W2387956917 hasConceptScore W2387956917C104317684 @default.
- W2387956917 hasConceptScore W2387956917C111335760 @default.
- W2387956917 hasConceptScore W2387956917C125593758 @default.
- W2387956917 hasConceptScore W2387956917C138885662 @default.
- W2387956917 hasConceptScore W2387956917C142613039 @default.
- W2387956917 hasConceptScore W2387956917C150194340 @default.
- W2387956917 hasConceptScore W2387956917C153911025 @default.
- W2387956917 hasConceptScore W2387956917C179926584 @default.
- W2387956917 hasConceptScore W2387956917C185592680 @default.
- W2387956917 hasConceptScore W2387956917C202751555 @default.
- W2387956917 hasConceptScore W2387956917C41895202 @default.
- W2387956917 hasConceptScore W2387956917C502942594 @default.
- W2387956917 hasConceptScore W2387956917C54009773 @default.
- W2387956917 hasConceptScore W2387956917C54355233 @default.
- W2387956917 hasConceptScore W2387956917C55493867 @default.
- W2387956917 hasConceptScore W2387956917C81885089 @default.
- W2387956917 hasConceptScore W2387956917C86803240 @default.
- W2387956917 hasConceptScore W2387956917C94581717 @default.
- W2387956917 hasLocation W23879569171 @default.
- W2387956917 hasOpenAccess W2387956917 @default.
- W2387956917 hasPrimaryLocation W23879569171 @default.
- W2387956917 hasRelatedWork W1552439524 @default.
- W2387956917 hasRelatedWork W2006063977 @default.
- W2387956917 hasRelatedWork W2223850258 @default.
- W2387956917 hasRelatedWork W2349844359 @default.
- W2387956917 hasRelatedWork W2353450976 @default.
- W2387956917 hasRelatedWork W2358318212 @default.
- W2387956917 hasRelatedWork W2359895488 @default.
- W2387956917 hasRelatedWork W2363171231 @default.
- W2387956917 hasRelatedWork W2365738802 @default.
- W2387956917 hasRelatedWork W2366671154 @default.
- W2387956917 hasRelatedWork W2366672705 @default.
- W2387956917 hasRelatedWork W2368551292 @default.
- W2387956917 hasRelatedWork W2371848815 @default.
- W2387956917 hasRelatedWork W2381336896 @default.
- W2387956917 hasRelatedWork W2385261995 @default.
- W2387956917 hasRelatedWork W2390857970 @default.
- W2387956917 hasRelatedWork W2391393633 @default.
- W2387956917 hasRelatedWork W2392940906 @default.
- W2387956917 hasRelatedWork W2409954956 @default.
- W2387956917 hasRelatedWork W3029385573 @default.
- W2387956917 isParatext "false" @default.
- W2387956917 isRetracted "false" @default.
- W2387956917 magId "2387956917" @default.
- W2387956917 workType "article" @default.