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- W2387967504 abstract "A construction AcrA pMD 18T was generated by inserting the sequence of 691 bp obtained by a PCR into pMD 18T vector and selecting the sense clones.The result showed that the cloned sequence coincides with the designed sequence by sequencing.This construction was digested with the same enzymes (HindⅢ and BamHⅠ) and ligated the pET28a vector. Then the plasmid AcrA pET28a was transformed to the competent cell of BL21(DE3).The sense clone was induced with IPTG.The expression of AcrA was observed on SDS PAGE." @default.
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- W2387967504 date "2003-01-01" @default.
- W2387967504 modified "2023-09-25" @default.
- W2387967504 title "Chemical Synthesis and Prokaryotic Experssion of Part of AcrA Gene" @default.
- W2387967504 hasPublicationYear "2003" @default.
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