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- W2387993014 abstract "Objective To investigate the expression of mouse Cpne5 gene mRNA in various tissues and its location in embryo.Methods After total RNA of main organs extracted,Cpne5 mRNA were detected by RT-PCR.The probe targeting Cpne5 mRNA was synthesized,and the Cpne5 mRNA expression of different gestational age mice embryos were detected by whole-mount hybridization in situ.Results Cpne5 mRNA was detected in ten kinds of tissues of adult mouse.High expression of Cpne5 mRNA was detected in cerebrum,cerebellum,testis and lung,but no signal in liver and muscle.In neonatal mice,the highest expression of Cpne5 mRNA was found in brains,followed by the tissues of eyes and kidney.The expression level in lung and liver were lower.The concentration of antisense probes transcripted by SP6 promoter was 100ng/μL,and the concentration of sense probes transcripted by T7 promoter was 10ng/μL.The hybridization experiments showed that Cpne5 mRNA was mainly expressed in telencephalon,diencephalon,mesencephalon and rhombomere of mouse embryo.Conclusion The mouse Cpne5 mRNA are highly expressed in brain tissues of neonatal and adult mice and located in brain in the process of mouse embryonic development." @default.
- W2387993014 created "2016-06-24" @default.
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- W2387993014 date "2013-01-01" @default.
- W2387993014 modified "2023-09-23" @default.
- W2387993014 title "Expression of Mouse Cpne5 Gene mRNA in Various Tissues and Its Location in Embryo" @default.
- W2387993014 hasPublicationYear "2013" @default.
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