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- W2388005003 abstract "【Objective】To investigate the inducing effects of brain derived neurotrophic factor(BDNF)on the differentiation of the rat fetal neural stem cells(rNSCs).【Methods】The rat fetal neural stem cells(rNSCs) were obtained by clonal culture and recultured on the medium containing different dosage of BDNF to induce the rNSCs to different descendants.Formation of cell clone was observed by inverted optical microscope,the ultrastructure of the cells were observed by transmission electron microscope and the expression of the glial fibrillary acidic protein(GFAP)were detected by immunocytochemical method.【Results】Small cell spheres were observed by the inverted optical microscope after 5 d culturing and the cell clones in different sizes were formed after 7 d culturing.Results obtained by the transmission electron microscope indicated that the descendant cells with the characteristics of neural stem cells and gliacyte-like cells.There were more astrocyte-like cells after the cells induced by BDNF for 7 d,the length of their processes was increased by a progressively increased dosage of BDNF,the effects were most prominent in 40 ng/ml BDNF(P0.05).Immunocytochemical analysis indicated that the GFAP granules were expressed in cytoplasm,and the positive rates were reached 86.1% in 40 ng/ml BDNF group.【Conclusions】The NSCs would be differentiated to astrocyte-like cells under the induction of BDNF." @default.
- W2388005003 created "2016-06-24" @default.
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- W2388005003 date "2008-01-01" @default.
- W2388005003 modified "2023-09-25" @default.
- W2388005003 title "The inducing effects of BDNF on the differentiation of the rat fetal neural stem cells" @default.
- W2388005003 hasPublicationYear "2008" @default.
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