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- W2388014107 abstract "The aim of this study was to determine whether plasmid pR ST98 encoding resistance to antimicrobial agents in Salmonella typhi could mediate the resistance to non-specific immunity (serum bacteriocidal activity and phagocytosis by macrophages) of the host bacteria. Three chromosomally isogenic strains,that is,plasmid containing wild-type S.typhi STpR ST98,plasmid artificially cured by SDS ST(R -) and plasmid reintroduced into the cured strain pR ST98/ST(R -) were used to compare for their in vitro resistance to the bactericidal activity to human,rabbit and guinea pig serum. With a standard recovery procedure (log dilutions of lysed cells),the ability of survival within the macrophage MΦJ 774A.1 of BALB/c mouse of the three bacteria were compared,and the ability of resistance to phagocytosis of these three strains were further assayed by labeling and analysis of the bacterium-phagocyte interaction by flow cytometry. It was found that plasmid pR ST98 could mediate the resistance to serum bacteriocidal activity of its host bacteria,and also could enhance the ability of S.typhi to grow within the cells as determined by the macrophage survival test. More ST(R -) strains were phagocytosed by macrophages then STpR ST98 and pR ST98/ST(R -),but the numbers of viable bacteria of ST ST98 and pR ST98/ST(R -) were higher than that ST(R -).The results indicate that pR ST98 not only mediates drug resistance,but also resistance to non-specific immunity." @default.
- W2388014107 created "2016-06-24" @default.
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- W2388014107 date "2004-01-01" @default.
- W2388014107 modified "2023-09-25" @default.
- W2388014107 title "Study on the Resistance to Non-Specific Immunity of the Host Bacteria Mediated by the Drug Resistant Plasmid" @default.
- W2388014107 hasPublicationYear "2004" @default.
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