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- W2388046670 abstract "Objective To apply HPLC-ESI-MS combining with NMR and UV technique for the analysis of the major constituents of the extract of Giant knotweed R. Methods The separation was performed on the Agilent C18(250 mm×4.6 mm,5 μm)column.The mobile phase was composed of 0.2%(w)acetic acid in aqueous solution(A)and acetonitrile(B),and the flow rate was 0.8 mL·min-1.The wavelength of the UV detector was set at 254 nm.The MS spectrometer was Thermo LCQ Fleet mass spectrometer. Results Seventeen compounds including of rutin,chrysophanol,6-OH-emodin,emodin,hyperoside,isoquercetin,trifolin,austragalin,polygonimitin B,chrysophanol,emodin-8-O-glucoside,neomangiferin,mangiferin quercetin,kaempferol I3-II8-biapigenin and apigenin from Giant knotweed R.were detected by HPLC-MS. Conclusions The method of complex enzyme extraction combined with the analysis by HPLC-ESI-MS has been proved to be effective,sensitive and intuitionistic.It can be used to analyze the micro constituent in G.knotweed.This method can provide the reference for the analysis and study of other Chinese medicine." @default.
- W2388046670 created "2016-06-24" @default.
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- W2388046670 date "2011-01-01" @default.
- W2388046670 modified "2023-09-25" @default.
- W2388046670 title "Simultaneous analysis of 17 compounds from the extract of Giant knotweed R. by HPLC-ESI-MS" @default.
- W2388046670 hasPublicationYear "2011" @default.
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