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- W2388082215 abstract "Objective To construct recombinant fowlpox virus (FPV) with foot and mouth disease virus (FMDV) and study its genetic stability and immunogenicity.Methods Insert the genes encoding capsid protein precursor P1-2A and protease 3C into FPV expression vector.Co-transfect chick embryo fibroblast (CEF) with the constructed recombinant plasmid pTUAL3CP1 and FPV 282E4 strain to obtain recombinant fowlpox virus vUTAL3CP1 by BrdU pressure screening.The vUTAL3CP1 was subcultured in CEF for 30 passages and tested for virulence and genetic stability.Immunize BALB/c mice with vUTAL3CP1 and determine serum specific antibody and neutralizing antibody.Results The target gene was amplified from vUTAL3CP1 by RT-PCR.IFA showed positive reaction of vUTAL3CP1-transfected CEF with specific antibody against FMDV .The virulence of vUTAL3CP1 was stable after subculture in CEF for 30 passages,and no deletion or variation of gene was observed. High titers of specific antibody and neutralizing antibody were induced in mice immumized with vUTAL3CP1.Conclusion Recombinant fowlpox virus vUTAL3CP1 was successfully constructed and showed good genetic stability and immunogenicity." @default.
- W2388082215 created "2016-06-24" @default.
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- W2388082215 date "2008-01-01" @default.
- W2388082215 modified "2023-09-26" @default.
- W2388082215 title "Construction,Genetic Stability and Immunogenicity of Recombinant Fowlpox Virus vUTAL3CP1 with Capsid Protein Precursor P1-2A of Foot and Mouth Disease Virus" @default.
- W2388082215 hasPublicationYear "2008" @default.
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