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- W2388227639 abstract "Objective: To construct the replication defective adenoviral vector carried sCD80-Linker-sCD40L fusion gene. Methods: The coding gene of human sCD80、sCD40L and IL-12b signal were cloned by RT-PCR from human peripheral blood mononuclear cells (PBMC). It was constructed IL-12bSig-sCD80-Linker-sCD40L fusion gene by overlap PCR, and used the AdEasy XL Adenoviral Vector System, which was product by Stratagene Corporation, to generate replication defective adenoviral vector carried the sCD80-Linker-sCD40L fusion gene.Results: DNA sequence analysis proved that we cloned the coding gene of human sCD80、sCD40L and IL-12b signal, and we constructed the replication defective adenoviral vector carried sCD80-Linker-sCD40L fusion gene. Its titer is 2×10 10cfu/ml. Conclusion: The replication defective adenoviral vector carried sCD80-Linker-sCD40L fusion gene was.successful constructed." @default.
- W2388227639 created "2016-06-24" @default.
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- W2388227639 date "2004-01-01" @default.
- W2388227639 modified "2023-09-25" @default.
- W2388227639 title "Construction of replication defective adenoviral vector carried sCD80-Linker-sCD40L fusion gene" @default.
- W2388227639 hasPublicationYear "2004" @default.
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