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- W2388253804 abstract "Objective Prepareing HolotoxinA1 as the reference substance and establishing HPLC-ELSD method to evaluate the quality of triterpene glycosides in Apostichopus japonicus from different areas.Methods The HolotoxinA1 was separated and purified by macroporous resin and HPLC;The structure of HolotoxinA1 was determined by MS and NMR.The HPLC-ELSD method was performed on a Hypersil Gold C18(150 mm×2.1 mm,3 μm),using acetonitrilewater(containing 5 mmol·L-1 ammonium acetate) as the mobile phase with gradient elution.Results The HolotoxinA1 was characterized by the MS and NMR and could be used as reference substance by the HPLC-ELSD analysis.The established method of HPLC-ELSD indicated that the calibration curve showed good linearity from 0.2 μg·mL-1 to 25 μg·mL-1(r=0.999) and the lower limit of detection was 0.05 μg·mL-1 at a signal to noise ratio of 3.Conclusion This HolotoxinA1 is one of the most abundant triterpene glycosides in Apostichopus japonicus.The Apostichopus japonicus from east coast of South Korea has the most HolotoxinA1(588.20 μg·g-1)and the Apostichopus japonicus from Fujian province has the least HolotoxinA1(21.33 μg·g-1) according to the analysis." @default.
- W2388253804 created "2016-06-24" @default.
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- W2388253804 date "2013-01-01" @default.
- W2388253804 modified "2023-09-24" @default.
- W2388253804 title "Isolation,preparation and analysis of triterpene glycoside Holotoxin A_1 in Apostichopus japonicus" @default.
- W2388253804 hasPublicationYear "2013" @default.
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