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- W2388367064 abstract "Several mutants of Rhizobium astragalus strain 107 with transposon Tn5 insertion fail to produce normal amount of extracellular polysaccharides, and induce on the host Astragalus sinicus L. only ineffective nodules void of the nitrogen-fixation activity. To investigate the possibility that R. astragalus Exo~+Nod~- mutants, which have lost their Sym-plasmid and are thoroughly unable to in.fect the host, might the Exo- mutants to invade the host, we co-inoculated the plants with both types of the mutants and found unexpectedly that the former type did not improved their symbiotic behavior with the help of Exo~+ Nod~- mutants while the latter occupied the nodules exclusively. The result indicate that the mutants which have lost their Sym-plasmid and their symbiotic activity may enter the plant cells after the nodule initiation was induced by the mutant defective in the EPS synthesis but containing the Sym-plasmid. Thus we conciude that certain genes located on the Symplasmid are involved only in the very early stages of the rhizobium infection process whereas the exo genes are necessary for the development of nodules.In other experiments in which the pSym-cured Exo~+Nod~- mutants of other three species of Rhizobium were applied to co-inoculate A. sinicus respectively with R. astragalus Nod~+ Exo~- mutants, only ineffective nodules were formed on the plant roots and the other species of bacteria were re-isolated from these nodules, indicating that the specificity requirement of the plants for the symbiotic bacteria had declined after the early recognition took place." @default.
- W2388367064 created "2016-06-24" @default.
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- W2388367064 date "1992-01-01" @default.
- W2388367064 modified "2023-09-25" @default.
- W2388367064 title "Two Classes of Genes are Involved in the Nodulation of Astragalus sinicui" @default.
- W2388367064 hasPublicationYear "1992" @default.
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