FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2388382717> ?p ?o ?g. }

• W2388382717 abstract "Dead Sea therapy is one of the oldest forms of treatment for skin disease and some chronic inflammatory diseases like arthritis and psoriasis. Much of the research to date has attributed the clinical effects of Dead Sea therapy to its mineral composition; mostly to magnesium salts. Magnesium salts, such as magnesium sulphate (Epsom salts), have long been used as a spa product and as a therapeutic to manage clinical conditions. The rationale of this PhD research was to understand the role of topically applied magnesium ions in epidermal integrity of human skin. We compartmentalized this research project into studying physiological, metabolic and proteolytic changes occurring at different layers of the skin. To begin with, we investigated the permeability of magnesium ions through stratum corneum. In Chapter 3, we developed a method to stain skin sections using mag-fura-2, a fluorescent dye that specifically binds to magnesium ions. When magnesium chloride (MgCl2) solution was topically applied on normal and tape stripped skin, we observed that it is possible for magnesium ions to permeate through stratum corneum. We also varied the concentration and time of exposure of MgCl2 solutions and found that at higher magnesium concentration, permeability is faster and penetration increases with progression of time. To further characterize permeability of the skin to magnesium, we blocked individual hair follicles using a novel method and subsequently treated skin with MgCl2 solution. We found that hair follicles significantly contribute towards the permeation of magnesium ions. In Chapter 4, the hypothesis tested was that magnesium accelerates barrier recovery. Experiments were conducted on volunteers who did not have any history of skin conditions. We caused stratum corneum disruption on their volar forearm through tape stripping and subsequently treated that defined region with different salt solutions. The parameters we considered for understanding barrier function were transepidermal water loss, skin hydration and skin pH over 96 hours with repeated treatment at 24-hour intervals. We found that treatment with a variety of salt solutions, including magnesium chloride did not aid in lowering the TEWL to levels pre-tape stripping and no changes to skin pH were observed. However, we observed that treating the skin with 1.9 M magnesium solution nearly 2 fold (p < 0.05) increased hydration at 6 hours, relative to baseline at time zero, whereas water treatment increased hydration only 1.2 fold, and the hydration returned to normal levels within 24 hours in both cases. Skin barrier formation is a continuous process and occurs as a result of differentiating epidermal cells subsequently leading to terminal differentiation. In the case of disrupted barrier, the epidermal cells differentiate to replenish the lost layers of stratum corneum. Therefore as an extension to Chapter 4, experiments in Chapter 5 involved understanding the metabolic changes occurring in all three layers of the epidermis – granulosum, spinosum, basale. We used multiphoton tomography coupled with fluorescent lifetime imaging microscopy to observe volunteers 0, 24 and 96 hours. Defined regions of volar forearm were treated with water or 1.9M MgCl2 solution on normal and tape stripped skin at 24-hour intervals over 96 hours. In all the three layers, we observed that redox ratio significantly decreased (p<0.05) immediately after tape stripping and significantly increased (p<0.05) after 24 hours in the magnesium treated site in granulosum and spinosum layer, when compared to the normal skin site. This increase is indicative of changes in metabolic activity in the granular layer, suggestive of signs of differentiation and cell proliferation. However, we did not observe any significant change in NAD(P)H/FAD lifetime nor in the ratio of nuclear area to total cellular area (N/C). In order to gain more understanding about the role of magnesium in terminal differentiation, as reported in Chapter 6 we studied the filaggrin (FLG) regulation under varying concentrations of salt solutions and due to stimulation by external factors. We found that treating the excised skin with high concentrations of magnesium and calcium chloride solutions resulted in increased levels of FLG after 30 minutes of treatment. The increase in FLG levels could be due to the interaction of divalent ions with the binding sites present in the profilaggrin, subsequently exposing the sites for cleavage by enzymes such as caspase-14. Also, inflicting damage to the epidermis in excised skin using 2,4-dinitrochlorobenzene (DNCB) and tape stripping increased FLG levels when incubated for 20 hours. Also, we found that treating the skin with these salt solutions prior to stimulating them with DNCB or TS did not allow FLG levels to peak above those observed with unstimulated skin. Increased levels of FLG as a result of salt treatment could have a possible role in preparing the epidermal cells for terminal differentiation eventually leading to barrier formation. We applied salt solution treatments on the volar forearm of human volunteers to measure physiological parameters and found a significant increase in hydration, but not skin pH when compared to the control site. This ensured that the changes observed in FLG levels are not due to pH changes. From these results we conclude that magnesium ions can penetrate through healthy skin with intact stratum corneum, with significant contribution from hair follicles. At high magnesium concentrations the permeation of Mg2+ into the epidermis increases with time and is significantly higher than the baseline concentration of Mg2+ in the epidermis of untreated controls after 15 minutes of exposure. Even though magnesium treatment caused an increase in skin hydration at 6 hours, we did not find effects on TEWL or skin pH. Magnesium treatment also increased the redox ratio of cells in granulosum and spinosum layer indicating changed metabolic activity. We also found that magnesium ions could be involved in proteolytic cleavage of profilaggrin preparing the epidermal cells for terminal differentiation and providing a possible preventive effect against external stimulants." @default.
• W2388382717 created "2016-06-24" @default.
• W2388382717 creator A5034839677 @default.
• W2388382717 date "2016-04-01" @default.
• W2388382717 modified "2023-09-27" @default.
• W2388382717 title "Effect of topical magnesium application on epidermal integrity and barrier function" @default.
• W2388382717 doi "https://doi.org/10.14264/uql.2016.160" @default.
• W2388382717 hasPublicationYear "2016" @default.
• W2388382717 type Work @default.
• W2388382717 sameAs 2388382717 @default.
• W2388382717 citedByCount "0" @default.
• W2388382717 crossrefType "dissertation" @default.
• W2388382717 hasAuthorship W2388382717A5034839677 @default.
• W2388382717 hasBestOaLocation W23883827172 @default.
• W2388382717 hasConcept C12554922 @default.
• W2388382717 hasConcept C127413603 @default.
• W2388382717 hasConcept C142724271 @default.
• W2388382717 hasConcept C178790620 @default.
• W2388382717 hasConcept C185592680 @default.
• W2388382717 hasConcept C2777459323 @default.
• W2388382717 hasConcept C2778370115 @default.
• W2388382717 hasConcept C42475967 @default.
• W2388382717 hasConcept C519063684 @default.
• W2388382717 hasConcept C543218039 @default.
• W2388382717 hasConcept C54355233 @default.
• W2388382717 hasConcept C71924100 @default.
• W2388382717 hasConcept C80107235 @default.
• W2388382717 hasConcept C86803240 @default.
• W2388382717 hasConceptScore W2388382717C12554922 @default.
• W2388382717 hasConceptScore W2388382717C127413603 @default.
• W2388382717 hasConceptScore W2388382717C142724271 @default.
• W2388382717 hasConceptScore W2388382717C178790620 @default.
• W2388382717 hasConceptScore W2388382717C185592680 @default.
• W2388382717 hasConceptScore W2388382717C2777459323 @default.
• W2388382717 hasConceptScore W2388382717C2778370115 @default.
• W2388382717 hasConceptScore W2388382717C42475967 @default.
• W2388382717 hasConceptScore W2388382717C519063684 @default.
• W2388382717 hasConceptScore W2388382717C543218039 @default.
• W2388382717 hasConceptScore W2388382717C54355233 @default.
• W2388382717 hasConceptScore W2388382717C71924100 @default.
• W2388382717 hasConceptScore W2388382717C80107235 @default.
• W2388382717 hasConceptScore W2388382717C86803240 @default.
• W2388382717 hasLocation W23883827171 @default.
• W2388382717 hasLocation W23883827172 @default.
• W2388382717 hasOpenAccess W2388382717 @default.
• W2388382717 hasPrimaryLocation W23883827171 @default.
• W2388382717 hasRelatedWork W1500279923 @default.
• W2388382717 hasRelatedWork W1965929400 @default.
• W2388382717 hasRelatedWork W1972320543 @default.
• W2388382717 hasRelatedWork W2047591096 @default.
• W2388382717 hasRelatedWork W2053399613 @default.
• W2388382717 hasRelatedWork W2060937316 @default.
• W2388382717 hasRelatedWork W2075554042 @default.
• W2388382717 hasRelatedWork W2103095051 @default.
• W2388382717 hasRelatedWork W2163452496 @default.
• W2388382717 hasRelatedWork W2212873700 @default.
• W2388382717 hasRelatedWork W2378525586 @default.
• W2388382717 hasRelatedWork W2399391072 @default.
• W2388382717 hasRelatedWork W2413142929 @default.
• W2388382717 hasRelatedWork W2517656667 @default.
• W2388382717 hasRelatedWork W2911009397 @default.
• W2388382717 hasRelatedWork W2946165852 @default.
• W2388382717 hasRelatedWork W3084025915 @default.
• W2388382717 hasRelatedWork W3135554565 @default.
• W2388382717 hasRelatedWork W3137255739 @default.
• W2388382717 hasRelatedWork W1966314452 @default.
• W2388382717 isParatext "false" @default.
• W2388382717 isRetracted "false" @default.
• W2388382717 magId "2388382717" @default.
• W2388382717 workType "dissertation" @default.