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- W2388438085 abstract "Objective: To establish a RP-HPLC method for simultaneous determination of maslinic acid,corosolic acid,betulinic acid,oleanolic acid and ursolic acid in the flower buds of Eugenia caryophyllata by ultrasonic wave aided method. Methods: The separation was achieved on a Kromasil C18column(4. 6 mm × 250 mm,5 μm) at 30 ℃ using methanol-water-phosphoric acid solution(86∶14∶0. 1) as the mobile phase at a flow rate of 1. 0 mL· min-1and 210 nm as the detection wavelength. Results: The linear ranges of maslinic acid,corosolic acid,betulinic acid,oleanolic acid,and ursolic acid were 0. 0488-1. 220 μg(r = 0. 9994),0. 0856-2. 140 μg(r = 0. 9996), 0. 1680-4. 200 μg(r = 0. 9999),0. 2024-5. 060 μg(r = 0. 9997) and 0. 2224-5. 560 μg(r = 0. 9999),respectively. The average recoveries(n = 6) were 100. 9%,98. 94%,98. 25%,99. 55%,and 101. 1%,and RSDs were 2. 0%,2. 0%,1. 5%,0. 39%,and 1. 4%,respectively. Conclusion: The methodology validation shows that The proposed method is accurate,simple and reliable,which is applicable for the determination of triterpenic acid in the flower buds of Eugenia caryophyllata." @default.
- W2388438085 created "2016-06-24" @default.
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- W2388438085 date "2014-01-01" @default.
- W2388438085 modified "2023-09-26" @default.
- W2388438085 title "Simultaneous determination of contents of five triterpenic acids in the flower buds of Eugenia caryophyllata by RP-HPLC" @default.
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