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- W2388454745 abstract "Objective:To construct the replication defective retroviral vectors carried sense or antisense RNA of transforming growth factor β1(TGFβ1) respectively.Methods:The bioactive peptide-coding gene of TGFβ1 were inserted into retrovirus pRevTRE in two directions by blunt end ligation. The different vectors were transferred into packaging cell line PA317 with lipofectamine, and harvested the replication defective retroviral vectors that integrated sense or antisense insert fragment, which were named as pRevTβ and pRevTβ-AS respectively. NIH3T3 cell were infectd by vectors to estimate the viral titre, and bladder cancer cell line EJ were infected to evaluate the integrating and expressing rates by Southern-blotting and semi-quantative RT-PCR.Results:The titre of pRevTβ and pRevTβ-AS were 0.84, 0.88×105CFU/ml differently, the integrating rate of vectors to EJ cells were 100%,the TGFβ1 mRNA levels of EJ cells that transferred pRevTβ and pRevTβ-AS were 1.89 and 0.35 respectively. Conclusion: The constucted retroviral vectors could coincide with the requestion of tumor cell transfection, integrate with tumor cells efficiently, and express effectively." @default.
- W2388454745 created "2016-06-24" @default.
- W2388454745 creator A5040197241 @default.
- W2388454745 date "2002-01-01" @default.
- W2388454745 modified "2023-09-23" @default.
- W2388454745 title "Construction and evaluation of replication defective retroviral vectors carried sense or antisense RNA of TGFβ1 respectively" @default.
- W2388454745 hasPublicationYear "2002" @default.
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