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- W2388481303 abstract "Objective To improve the traditionary procedure of in situ hybridization in order to enhance signal intensity and speciality,then to analyze the expression of Pax3(pairedbox3) in Chick Embryo spinal cord with the improved method.Methods Molecular cloning of Pax3 into pCR-TopoII,Pax3 cRNA antisense probes synthesis with sp6 RNA polymerase in vitro,and the distribution of Pax3 mRNA was analyzed by the improved in situ hybridization.Results Pax3 cRNA probe was obtained.Pax3 mRNA was found that it appeared as region specifically according to the improved method.Conclusion The improved method enhance remarkably the signal speciality of in situ hybridization.The expression of Pax3 mRNA appeares as region specifically in Chick Embryo spinal cord,which indicates that Pax3 plays certain role during the development of spinal cord." @default.
- W2388481303 created "2016-06-24" @default.
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- W2388481303 date "2008-01-01" @default.
- W2388481303 modified "2023-09-27" @default.
- W2388481303 title "Expression of Pax3 gene in Chick Embryo spinal cord analyzed with the improved in situ hybridization" @default.
- W2388481303 hasPublicationYear "2008" @default.
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