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- W2388481573 abstract "Objective To investigate the activity of skin Keratinocytes and Fibroblasts influenced by medium with different concentrations of serum in vitro. Methods Keratinocytes and Fibroblasts were cultured in vitro, inoculating into 96 Orifice in accordance with 3 × 105 / ml density, applied with K-SFM Media with different serum to culture, and activity of cells observed by the method of MTT colorimetric. Results Application of K-SFM +10% FBS medium and DMEM +10 % FBS for the control group to culture.Fibroblasts , the activity of the cells are the same (P 0.05); Application of K-SFM +1% FBS medium and K-SFM for the control group to culture keratinocytes, the activity of the cells are the same (P 0.05). Conclusions Construction of artificial skin model in vitro, Keratinocytes and Fibroblasts can be plus1% FBS, 10% FBS to the K-SFM respectively at culture medium ." @default.
- W2388481573 created "2016-06-24" @default.
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- W2388481573 date "2009-01-01" @default.
- W2388481573 modified "2023-09-25" @default.
- W2388481573 title "Investigate the activity of skin Keratinocytes and Fibroblasts influenced by Different culture conditions in vitro" @default.
- W2388481573 hasPublicationYear "2009" @default.
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