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- W2388536757 abstract "Aim To explore whether Na+,K+-ATPase involves in the decrease of calcium transient in cardiac myocytes from guinea pig with chronic heart failure(CHF).Methods The CHF model in guinea pig was produced by constricting the descent aorta(CDA) or subcutaneously injecting isoproterenol(ISO).Left ventricular myocytes were enzymatically isolated.Inorganic phosphorus method was used to determine cardiac sarcolemmal Na+,K+-ATPase activity.Then the expression of Na+,K+-ATPase α1 and α2 subunit in mRNA level and in protein level were detected with RT-PCR and Western blot,respectively.Results The expressions of Na+,K+-ATPase α1 and α2 subunit in mRNA level decreased in CDA CHF guinea pigs,α1 subunit also decreases but α2 subunit unchanges in ISO CHF guinea pigs.Both the Na+,K+-ATPase activity and the expression of Na+,K+-ATPase α1 subunit in protein level were unchanged in the two CHF guinea pigs.Conclusion Na+,K+-ATPase may not involve in the decrease in calcium transient induced by CHF." @default.
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- W2388536757 date "2009-01-01" @default.
- W2388536757 modified "2023-09-26" @default.
- W2388536757 title "Na~+,K~+-ATPase may not involve in the decrease of calcium transient in cardiac myocytes from guinea pig with chronic heart failure" @default.
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