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- W2388556673 abstract "To select the genes of trans-factors involved in the regulation of the synthesis of astaxanthin, the total RNA was extracted from H.pluvlialis, the purified mRNA was reversed to cDNA, and a cDNA expression library was constructed using λ ZAP Express vector from motile cells of Haematococcus pluvlialis. The test result showed the titer of the cDNA expression library was 5.1× 105 pfu/ml and the efficiency of recombination was 100%. Using PCR to test the quality of the constructed library, the results showed that the average size of the cDNA insert fragments was 1.7 kb. The constructed cDNA Expression library from H. pluvialis has laid foundation for the research of metabolic engineering of astaxanthin." @default.
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- W2388556673 date "2006-01-01" @default.
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- W2388556673 title "Construction and Identification of cDNA Expression Library from Haematococcus pluvialis" @default.
- W2388556673 hasPublicationYear "2006" @default.
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