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- W2388618612 abstract "Objective To observe the protective and therapeutic effects of nerve growth factor (NGF) on β Amyloid25 35(Aβ25 35)-induced hippocampal neuron injury.Methods Hippocampal neurons obtained from 15 new born SD rats within 3 days were cultured in neural basal medium+B27 factor for 7 days,then Aβ25 35(10μg/ml) was added before and after addition of NGF(10μ/ml) and equal amount of culture fluid separately in two groups.MTT detection and β tublin ChAT immunohistochemistry were used to determine the integrity of hippocampal neurons,cell viability and ChAT positive neurons.Results β tublin immunohistochemistry demonstrated the integrated hippocampal cells.MTT and ChAT immunohistochemistry showed that the viability of hippocampal cells and the number of ChAT positive neurons were decreased significantly in Aβ25 35 treated group.Conclusion NGF can effectively prevent and mildly improve the injury of hippocampal neurons induced by Aβ25 35.NGF may have protective and therapeutic effects for Alzheimer's disease." @default.
- W2388618612 created "2016-06-24" @default.
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- W2388618612 date "2002-01-01" @default.
- W2388618612 modified "2023-09-25" @default.
- W2388618612 title "Effects of nerve growth factor on Aβ_(25. 35) induced hippocampal neuron injury" @default.
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