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- W2388618888 abstract "Serum of patients infected by HCV and without infected by HCV collected from three hospital were detected by three HCV EIA reagents.Positive and negative serum validated by all the three reagents were chose respectively and then detected by RNAhyb PCR reagents.Furthermore,after detected by RIBA reagents,parts of them was achieved as reference for the quality control of the proteinchip.The qualified proteinchip was identified by HCV standard of National Institute for the Pharmaceutical and Biology Products(NICPBP).The quality of the proteinchip was further evaluated through detec-tion of490clinical samples.30serum with15positive and15negative were achieved from240collected serums of pa-tients infected by HCV and considered as reference of the quality control of the proteinchip for the detection of different HCV antibody.Through detected with80standard of NICPBP,The results shows the positive and negative conformity ra-tios were39/40and40/40,27/40and40/40,26/40and39/40,19/40and40/40,2/40and40/40for combined anti-body,core antibody,antibody to NS3,antibody to NS4,antibody to NS5respectively compared with ELISA.The results of clinical sample show that the conformity ratio of combined antibody and different antibody of the proteinchip were99.5%and97.1%respectively compared with ELISA.The unconformity samples were validated by RIBA reagents and the result shows the conformity of proteinchip with RIBA reagents was high consist.The results of296clinical samples without HCV infected show negative all.The prepared reference for quality control of protein-chip for the detection of different HCV antibody can be used as quality control for the production of the proteinchip.The produced proteinchip can pass the demand of standard of NICPBP and can be used in clinical detection." @default.
- W2388618888 created "2016-06-24" @default.
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- W2388618888 date "2004-01-01" @default.
- W2388618888 modified "2023-09-25" @default.
- W2388618888 title "Establish and validation of reference for quality control of protein-chip for different HCV antibody detection" @default.
- W2388618888 hasPublicationYear "2004" @default.
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