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- W2388714162 abstract "Objective:To develop fluorescence quantitative reverse transcription polymerase chain reaction (FQ-RT-PCR) for quantitative detection of transforming growth factor β1 (TGF-β1) mRNA in peripheral blood mononuclear cell of patients with diabetes mellitus. Methods:the PBMC was separated, and the total RNA was extracted with Trizol lysate, and then the cDNA was transcripted, the SYBR GREEN- I was used in the amplification system for quantitative detection of TGF-β1 mRNA. Results:The FQ- RT-PCR could detect as low as 105 copies per milliliter recombined plasmid standard,the linear range was from 105 to 1011 copies per milliliter, and the coefficient variation were 2.27%～2.56% and 4.78%～5.22% in intra and inter-assay respectively. The levels of TGF-β1 mRNA in PBMC of patients with diabetes mellitus were significantly higher than that of healthy control(sP0.05).Conclusion:FQ-RT-PCR is a simple and rapid method as the primary application of the screening model for transforming growth factor β1 mRNA that eliminates the post-PCR processing steps." @default.
- W2388714162 created "2016-06-24" @default.
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- W2388714162 date "2006-01-01" @default.
- W2388714162 modified "2023-09-28" @default.
- W2388714162 title "Establishment and preliminary application of the screening model for transforming growth factor β_1 mRNA by fluorescence quantitative reverse transcription polymerase chain reaction" @default.
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