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- W2388730477 abstract "Objective To observe the effect of Survivin gene interference and overexpression on apoptosis of human breast cancer MCF-7 cells.Methods MCF-7 cells were transfected with shRNA interfering plasmid and overexpression plasmid pcDNA3.1-GFP-Survivin in mediation of liposome,using those untransfected as blank control and those transfected with liposome as negative control,and observed for transfection efficacy by fluorescent microscopy,for transcription level of Survivin mRNA by fluorescent quantitative RT-PCR,and for apoptosis rate by MTT method.Results The transfection efficacies of MCF-7 cells 48 h after transfection with Survivin gene RNAi plasmid and overexpression plasmid pcDNA3.1-GFP-Survivin were 50% ~ 60%.The transcription level of Survivin mRNA in shRNA plasmid transfection group was significantly lower,while that in overexpression plasmid transfection group was significantly higher,than those in blank and liposome control groups(P 0.05).However,the apoptosis rate of MCF-7 cells in shRNA plasmid transfection group was significantly higher,while that in overexpression plasmid transfection group was significantly lower,than those in blank and liposome control groups(P 0.05).Conclusion Survivin gene showed a certain inhibitory effect on apoptosis of MCF-7 cells,which might be used as a candidate gene for biotherapy of human breast cancer." @default.
- W2388730477 created "2016-06-24" @default.
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- W2388730477 date "2013-01-01" @default.
- W2388730477 modified "2023-09-22" @default.
- W2388730477 title "Effect of Survivin gene interference and overexpression on apoptosis of human breast cancer MCF-7 cells" @default.
- W2388730477 hasPublicationYear "2013" @default.
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