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- W2388769759 abstract "Objective To establish real-time fluorescence polymerase chain reaction(PCR)for HIV provirus detection.Methods HIV-1 provirus DNA was extracted after 8E5 cells were quantified.A fragment of late reverse transcripts(full-length)was tested by real-time fluorescence PCR.Results The quantitative real-time PCR assay was sensitive and specific.1 copy of HIV FL could be consistently detected using this assay.Conclusion The method of HIV provirus detection was established successfully,which lay the foundation for further study on screening and verifying latent infected cells,evaluating the therapeutic efficacy of antiretroviral treatment." @default.
- W2388769759 created "2016-06-24" @default.
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- W2388769759 date "2008-01-01" @default.
- W2388769759 modified "2023-09-28" @default.
- W2388769759 title "Establishment of Real-time Fluorescence Polymerase Chain Reaction Based on the TaqMan Probes for Detection of HIV Provirus" @default.
- W2388769759 hasPublicationYear "2008" @default.
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