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- W2388804942 abstract "AIM: To explore whether there are some similar or different properties between endothelial progenitor cells (EPCs) derived from human peripheral blood and mature vessel wall endothelial cells in antigen expression, cell morphology, proliferation potential, and in vitro and in vivo vasculogenesis. METHODS: Mononuclear cells were isolated by density-gradient centrifugation and incubated onto fibronectin-coated dishes in endothelial me-dium in the presence of vascular endothelial growth factor. After cultivation, cells were sequentially replanted by allowing cells to adhere to tissue culture dishes during two successive 2-h incubations in order to remove nonadherent cells. Medium was changed every other day and the cultivation was made continually until the late clones generated. At the same time, human umbilical vein endothelial cells (HUVECs) were cultured and same biological properties were compared with EPCs. Flow cytometry analysis was used to evaluate cell surface antigen expression and cells binding ulex europaeus agglutinin (UEA)-1 and up-taking DiI-labeled acetylated low density lipoprotein (DiI-Ac-LDL) were detected by direct fluorescence staining. Moreover, cell proliferation potential was estimated by calculating the number of population doubling times and the capacities of in vitro and in vivo vascular genesis were assessed by plating cell onto collagen gels and implanting the cellularized gel into nude mice. RESULTS: EPCs emerged until 21 to 28 d after cultivation and exhibited coble-stone like typical endothelial cells. When compared with HUVECs, EPCs expressed higher levels of CD36 and KDR (P0.01), but there were no significant differences between the 2 kinds of cells in CD146 expression, cells binding UEA-1, and up-taking DiI-Ac-LDL. During the 100-d in vitro cultivation, EPCs and HUVECs reached 46 and 25 divisions respectively. Remarkably, only cells originated from EPCs had the ability to generate tube-like structures when seeded onto collagen gels or transplanted into nude mice. CONCLUSION: EPCs derived from human peripheral blood retain the complete biological properties of stem/progenitor cells in spite of exhibiting the phenotypic and morphologic characteristics of mature endothelial cells." @default.
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- W2388804942 date "2008-01-01" @default.
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- W2388804942 title "Comparison of biological properties between human peripheral blood derived endothelial progenitor cells and mature vessel wall endothelial cells" @default.
- W2388804942 hasPublicationYear "2008" @default.
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