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- W2388829603 abstract "In single enzyme RT PCR,Taq enzyme plays as roles of both reverse transcripase and DNA polymerase.The reaction conditions of single enzyme RT PCR was researched in this paper in order to detect PSTV rapidly.The results indicated that when the anneal temperature was 37 ℃ and the circles were more than 10 times in reverse transcription phase,a relative band of PSTV was obtained by PCR which could be proved using nested set PCR.Single enzyme RT PCR was simple and low cost,so it could play on important role for the detection of PSTV." @default.
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- W2388829603 date "2002-01-01" @default.
- W2388829603 modified "2023-09-28" @default.
- W2388829603 title "Detection of Potato Spindle Tuber Viroid by Single-enzyme RT-PCR" @default.
- W2388829603 hasPublicationYear "2002" @default.
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