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- W2388834920 abstract "Objective To explore the methods of construction of mtDNAdeletion cell line (ρ0 cells) and provide the cell model in the followup study.Methods The mtDNAfree ρ0 cell line used in this study was generated by incubating SHSY5Y cells in growth media containing 5 or 7.5 μg/ mL ethidium bromide (EtBr) for longterm incubation.Identification of ρ0 cells were measured by electron microscopy and mtDNA PCR amplification.Results Complete transformation of SHSY5Y cells to mtD-NAdeletion ρ0 cells required 95 days of incubation in 7.5 μg/ mL ethidium bromide.Mitochondria in ρ0 cells became swollen and round, had grossly reduced and distorted cristae and essentially empty matrix giving it a ‘ghostlike' appearance.The target band was not seen after mtDNA PCR amplification.Conclusion Succeeding in construction of ρ0 cells will provide cell model for the future indepth research." @default.
- W2388834920 created "2016-06-24" @default.
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- W2388834920 date "2010-01-01" @default.
- W2388834920 modified "2023-09-22" @default.
- W2388834920 title "Construction and identification of mtDNA deletion cell line" @default.
- W2388834920 hasPublicationYear "2010" @default.
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