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- W2388861602 abstract "Objective: To obtain the procaryotic expression vector containing the antifreeze protein gene AFP. Methods: By directional cloning method, AFP gene fragment was cloned into the expression vector pIN Ⅲ OmpA3 and the recombinant plasmid DNA was identified with enzyme digestion.First, splice out the intron of the antifreeze protein gene, then induce its expression with IPTG, and finally determine the thermal hysteresis activity of the purified protein.Results: AFP gene fragment was cloned into the expression vector and we obtained the antifreeze protein successfully. Conclusion: The recombinant expression plasmid pIN Ⅲ OmpA3 is constructed successfully, which lays a good basis for factory producing antifreeze protein in the future." @default.
- W2388861602 created "2016-06-24" @default.
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- W2388861602 date "2001-01-01" @default.
- W2388861602 modified "2023-09-25" @default.
- W2388861602 title "Construction vector and effcient expression of antifreeze proteins gene" @default.
- W2388861602 hasPublicationYear "2001" @default.
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